Coding

Part:BBa_K3735016

Designed by: Jiandong Jiang   Group: iGEM21_NAU-CHINA   (2021-10-01)


Coding sequence

In order to improve the quality of SeNPs and improve the reduction efficiency, we hope to link those standard parts and then, two genes express at the same time. This part consist of low intensity SefA and middle intensity ssuE. After verifying the function of SefA and ssuE separately, we integrate them and obtain nine combinations of different promoter strengths. And the low SefA and the low ssuE Can produce the best SeNPs.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 3099
    Illegal NheI site found at 3122
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1537
    Illegal BglII site found at 2854
    Illegal BglII site found at 3288
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 329
    Illegal NgoMIV site found at 644
    Illegal NgoMIV site found at 770
    Illegal NgoMIV site found at 1151
    Illegal NgoMIV site found at 1574
    Illegal NgoMIV site found at 2675
    Illegal NgoMIV site found at 2924
    Illegal AgeI site found at 671
    Illegal AgeI site found at 1217
    Illegal AgeI site found at 1373
    Illegal AgeI site found at 1514
    Illegal AgeI site found at 1937
    Illegal AgeI site found at 2294
    Illegal AgeI site found at 2465
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None